Characterisation of tissue factor-bearing extracellular vesicles with AFM: comparison of air-tapping-mode AFM and liquid Peak Force AFM

Characterisation of tissue factor-bearing extracellular vesicles with AFM: comparison of air-tapping-mode AFM and liquid Peak Force AFM

INTRODUCTION Extracellular vesicles (EVs) are shed from cells and carry markers of the parent cells. Vesicles derived from cancer cells reach the bloodstream and locally influence important physiological processes. It has been previously shown that procoagulant vesicles are circulating in patients' fluids. These EVs are therefore considered as promising biomarkers for the thrombotic risk. Becau...

Tapping Mode AFM Imaging for Functionalized Surfaces

A Study of Tapping Mode Trajectories in an AFM

Abstract In an effort to aid in the understanding of the nonlinear dynamics of the trajectories followed by an Atomic Force Microscope (AFM), I have written an interactive java applet simulator using a simple inelastic billiard model developed by Cvitanović and Paškauskas. We study the trajectories followed by the tip of the AFM as a function of its physical parameters and initial conditions. T...

AFM in mode Peak Force applied to the study of un-worn contact lenses.

Contact lenses (CLs) are of common use and the biocompatibility, topography and mechanical properties of the used materials are of major importance. The objective of this contribution is to apply the AFM in mode Peak Force to obtain surface topography and mechanical characteristics of un-worn CLs of different materials. One material of hydrogel, two of siloxane-hydrogel and one of rigid gas-per...

Modeling Time-resolved Interaction Force Mode Afm Imaging